The loss of DLG2 isoform 7/8, but not isoform 2, is critical in advanced staged neuroblastoma

Abstract Background Neuroblastoma is a childhood neural crest tumor showing large clinical and genetic heterogeneity, one form displaying 11q-deletion very aggressive. It has been shown that results in decreased expression of DLG2 , gene residing the deleted region. number different isoforms with main difference presence or absence L27 domain. The domain containing DLG proteins can complexes CASK/MPP LIN7 protein family members, which will control cell polarity signaling. Methods We evaluated for their differently INSS staged neuroblastoma from publically available data primary tumors, we included two distinct DLG1 N-terminal transcript encoding domains expression. Functionality LIN7A were 11q-deleted line SKNAS. Results In only expressed: isoform 2 7/8. Using array could determine higher members contain correlated to better survival prognosis. Whilst showed decrease both increased stage, full length transcripts DLG2-isoform 7/8 high stage neuroblastoma. show encoded by 7 bind LIN7A, this reduced proliferation viability, BAX/BCL2 ratio indicating apoptosis. Conclusion have provided evidence but not lacking disrupted neuroblastoma, particular aggressive subsets tumors. complete allows binding signaling, thus affecting cancer viability.